dear colleagues:
In my laboratory, I often receive turbid patients'samples like
"milk",such as "triacylglycerol>5mmol/L".When I determined creatinine,
total protein, albumin, glucose, there were interferences in this
situation for turbid absorbence.To eliminate this interference,the
traditional method was extraction lipoid from organic compound,then
mearsured them.This method is so tedious that many laboratories do not
adopt it.
Piero Fossati et found the solution made of nonionic ethoxylated linear
secondary alcohol and sodium cholate could eliminate this
interference.The author thought twenty milky,fresh, hyperlipemic human
sera containing triacylglycerol >5 mmol/L(range 5-23 mmol/L) were mixed
with above solution,subsequently the absorbance changes were monitored
at 340 nm.Within 2 min,the absorbance values for all the samples tested
were similar to those of clear normal sera.For frozen-thawed
hyperlipemic sera, a longer thime (5-10min) was needed for complete
clarification. [clin-chem.40/1,130-137(1994)].This method can be used in
automatic analyzer without manual steps.
My question is how to purchase these materials (nonionic ethoxylated
linear secondary alcohol and sodium cholate)? and are there other
simple faster methods to eliminate the interferences of turbid sample
in automatic analyzer(such as Hitachi 717)?
Sincerely
rock shi
email address: [log in to unmask]
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