Dear colleagues,
These days, we have got an anticoagulated blood sample for molecular
diagnostics (detection of HFE mutations in suspected haemochromatosis)
after a transfusion of red blood cells had been perfomed.
I wonder if donor leukocyte contamination is a serious problem in
PCR-based diagnostics after the transfusion of red cells. Our
transfusion medicine colleagues told me that exclusively
leukocyte-reduced red blood cells are given at our Institution with a
leukocyte count of less than 1 million cells per unit red blood cells
(which is about 250 ml volume).
How long does it take these leukocytes to disappear from the blood in
the recipient? Should we request another sample and if so, when? Is
anyone aware of published evidence which might be of value?
Thank you very much in advance for your input.
Dr. med. Michael Steiner
University of Rostock
Institute of Clinical Chemistry & Pathobiochemistry
Ernst-Heydemann-Str. 6
18057 Rostock
Germany
fax: +49/381/494-7672
email: [log in to unmask]
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