Regarding the clearing of grossly lipaemic samples we have recently
investigated four methods. This was presented at the AACB (Australasian
Association of Clinical Biochemistry) Annual Scientific Meeting last year.
The methods reviewed were ultracentrifugation, trichlorotrifuoroethane
(which we used to know as SeroClear, and are also having trouble sourcing)
and 2 commercial products Cleanascite (Astral) and LipoClear (Statspin Inc).
Ultracentifugation remains the gold standard, and Cleanascite was
unsatisfactory both due to capacity and interferences with some analytes.
TrichloroTrifluoroethane and LipoClear were both quite good with minor
effects on some analytes. Lipoclear shows this in its product information
and recommends labs checking for themselves.
Interestingly Lipase (by clearing tubidity) was the worst analyte in all
methods, and an important one in lipaemic samples, and the new chromogenic
method (from Roche) is essentially impervious to lipaemia.
I would be happy to send a summary of our data as an attachment to
individual e-mails upon request.
Yours,
Graham Jones
Staff Specialist in Chemical Pathology
St Vincent's Hospital Sydney
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