Peter Vervaart wrote
> A quick and simple test we used to use was to measure the "Hb" with a
> urine dip stick (Trinder based) before and after filtration through a 30
> 000 MWt cut off filter.
>
> If the test was positive in both urines there is myoglobin present, if
> only positive in the first unfiltered urine it is haemoglobin. If negative
> in both then neither is present.
>I investigated this method some years ago with a view to
introducing it into routine use.
We added blood to urine samples to look at the effects of high
haemoglobin levels and found that the selectivity of the filters
was not good enough to prevent enough haemoglobin passing
through the filter to give a false positive myoglobin.
We considered trying a second filtration but went for an
immunological test instead.
Mike Collins
Mike Collins MLSO3
Clinical Biochemistry & Immunology
The General Infirmary at Leeds,
Old Medical School, Thoresby Place
Leeds LS1 3EX, UK
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44 (0) 113 392 2915
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