I expect that David Hunane is me. However I expect the assay is no longer
available at the London. Dr Anthony Norden at Chase Farm, Enfield also has
an assay available. Antibodies and calibrant are commercially available
through Dakopatts. The problem with a light scattering assay on the Cobas
is that without latex enhancement it is unlikely to be sensitive enough for
clinical use. An ELISA is probably the best method. I would hope though
that more commercial manufactures will develop assays soon as RBP is
probably the best available marker of proximal tubular damage that we have
currently available, certainly better than a1m.
Regards
Dr David Newman
Consultant Scientist & Scientific Director
SW Thames Institute for Renal Research
Tel: (181) 296 3698
Fax: (181) 644 8257
----------
> From: Dr Sachidanandam Vivekanandan <[log in to unmask]>
> To: Elisabeth Haschke-Becher <[log in to unmask]>
> Cc: [log in to unmask]
> Subject: Re: Retinol binding protein
> Date: 25 May 1999 14:03
>
>
> There are 3 methods available for the Retinol Binding Protein
> measurement to my knowledge, two in house methods & one commercial Elisa
> method;
> One of the in-house methods (?light scattering) is being used by
> C.P.Price group (Dave Hunane) & the other is being developed/ used
> by Sue Twyman at Southampton District General Hospital at Southampton,
> UK; This is also ? light scattering on Behring
> In addition to these two, there is a commercial Elisa method used in our
> Hospital by Dr.Neil Dalton group (paediatrics Natl Kidney research lab;
> Phone 0171-955-5000 Ex:4502) with a sensitivity of ~0.5 microgm/L in
> contrast to ~5 microgm/L by the light scattering methods.
>
> You can contact these groups for a lead
>
> Hope this is useful
> Regards
> Vivek
> Clinical Chemistry
> Guy's Hospital
> London
> On Tue, 25 May 1999, Elisabeth Haschke-Becher wrote:
>
> > Dear collegues!
> > Does anybody know a method for retinol binding protein, which can be
> > analysed on a Cobas FARA or Hitachi 717/917?
> > Thanks for all information
> >
> > Dr. Elisabeth Haschke-Becher
> > Laboratoire Central de Chimie Clinique
> > Centre HospitalierUniversitaire Vaudois
> > Bugnon 46
> > CH - 1011 Lausanne
> > Tel: 0041/21-3144129
> > Fax.: 0041/21-3144288
> >
>
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