We now use the cryohemolysis test which is much more sensitive and
specific than the osmotic fragility test for the screening of
spherocytosis. It is not inflenced by auto-immune hemolysis, for example.
The method is described in:
Romero R, Poo JL, Robles JA, Uriostegui A, Vargas F, Majluf-Cruz A.
Usefulness of cryohemolysis test in the diagnosis of hereditary
spherocytosis. Arch Med Res 1997;28:247-51.
Frederic Cotton
PharmD, Specialist in Clinical Pathology
Hôpital Erasme - Université Libre de Bruxelles
"R.W. Wulkan" wrote:
> Recently, we modified our osmotic fragility test according to
> H.C.Godal et al. Scand J Haematol 1980;25:107-12,
> which requires only four tubes with NaCl solutions instead of twelve,
> thereby limiting the amount of labour.
> Our concern is that by having points which lie mainly in the
> straight part of the curve, we will miss deviating forms
> (e.g. flatter curve than usual). Is there anyone who can tell us the
> clinical significance of such flattened curves, the frequency and
> associated diseases? Thanks for your comment.
>
> Raymond Wulkan
> Zuiderziekenhuis
> Rotterdam
> The Netherlands
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