A quick and simple test we used to use was to measure the "Hb" with a urine
dip stick (Trinder based) before and after filtration through a 30 000 MWt
cut off filter.
If the test was positive in both urines there is myoglobin present, if only
positive in the first unfiltered urine it is haemoglobin. If negative in
both then neither is present.
Hope this is useful
Peter V.
Peter Vervaart BAppSci MAIMS FAACB
Senior Scientist, Gastroenterology and Nutrition
Department of Clinical Biochemistry
Women's and Children's Health Care Network
Flemington Road
Parkville, Victoria, 3052
Australia
Phone: 613-9345 5906
Fax: 613-9349 1819
WWW: http://www.rch.unimelb.edu.au\biochem\gastro
----- Original Message -----
From: Mike Addison <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, October 14, 1999 9:13 AM
Subject: Haemaglobinuria or myoglobinuria
> In my lab spectrophotometric differentiation of Hb or myoglobin in
> urine is unreliable. If we detect haem in urine we use serum CK to
> differentiate. This is OK in most instances but occasionally we are
> left without an answer. Are there any quick ways others have for
> deciding which haem protein is present in urine?
>
> Mike Addison
> Dr G.Michael Addison
> Royal Manchester Children's Hospital
> Pendlebury
> Manchester M27 4HA
> United Kingdom
>
> Tel 0161-727-2250(AM)or 0161-220-5342(PM)
> FAX 0161-727-2249
> Email [log in to unmask]
>
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