We have experienced a strange behaviour of Relion 3.1 post-processing
filtering. Our map at 2.5A FSC estimated resolution is filtered to
around 2A in the post-processing job.
We noticed this by the averaged power spectrum, where the signal has
sharp drop not at 2.5 but at 2A. When we do the sample post-processing
job in Relion3.0, the FSC resolution and power spectrum correlates.
Have anybody seen anything similar. Is there any cure for that?
Thank you for your advice
Tomas
PS: When we filter ad hoc, the b factors get positive values.
--
Dr Tomas Kouba
EMBL Grenoble
71 avenue des Martyrs
CS 90181
38042 Grenoble Cedex 9
France
Tel: +33 476 209 478
e-mail: [log in to unmask]
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