Sorry, silly mistake - I forget enzymatic exists sometimes...
We're seeing this on the Jaffe method. I've had a couple of direct responses saying others have seen something identical - negative bias on lipaemic samples, and if you leave it for a day or so then the problem just disappears (even though the sample is not dramatically less lipaemic). No obvious correlation with anything so far. Roche are investigating of course. Has been useful to hear this is not just affecting us!
Thanks to all responses,
Alan.
Dr Alan Dodd, Principal Clinical Scientist (Research).
Biochemistry, Prince Charles Hospital, Merthyr Tydfil
On Tue, 3 Sep 2019 00:29:05 +0100, Gavin Murdock <[log in to unmask]> wrote:
>Hi
>
>I've not seen this (doesn't mean it's not happening).
>
>Can you clarify whether this is the Jaffe or enzymatic creatinine reagent
>and what the median value for the L-index of affected samples is? Roche use
>intralipid to evaluate the robustness of the assay to interference from
>turbidity - I guess it is possible that this is a poor surrogate for
>triglycerides.
>
>Do the affected patients have any medication or pathology in common? Do you
>run IQC/EQA for your serum indices?
>
>Thanks,
>Gavin Murdock
>
>On Mon, 2 Sep 2019 at 16:18, Alan Dodd <
>[log in to unmask]> wrote:
>
>> Hello,
>> We've been seeing small but significant numbers of samples with creatinine
>> affected by lipaemia, causing a variable negative bias at lipaemic index
>> values far below the official threshold. Removing the lipaemia with hard
>> centrifugation seems to resolve the bias. We're seeing it on both our
>> sites, on both 6000 and 8000 Roche platforms, so we are curious to know if
>> anyone elsewhere has seen a similar issue?
>>
>> Thanks,
>> Alan.
>>
>> Dr Alan Dodd, Principal Clinical Scientist (Research).
>> Biochemistry, Prince Charles Hospital, Merthyr Tydfil
>>
>> ------ACB discussion List Information--------
>> This is an open discussion list for the academic and clinical community
>> working in clinical biochemistry.
>> Please note, archived messages are public and can be viewed via the
>> internet. Views expressed are those of the individual who posts and they
>> are solely responsible for all message content. The ACB does not monitor
>> posts.
>>
>> ACB Web Site
>> http://www.acb.org.uk
>> Green Laboratories Work
>> http://www.laboratorymedicine.nhs.uk
>> List Archives
>> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
>> List Instructions (How to leave etc.)
>> http://www.jiscmail.ac.uk/
>>
>
>------ACB discussion List Information--------
>This is an open discussion list for the academic and clinical community working in clinical biochemistry.
>Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual who posts and they are solely responsible for all message content. The ACB does not monitor posts.
>
>ACB Web Site
>http://www.acb.org.uk
>Green Laboratories Work
>http://www.laboratorymedicine.nhs.uk
>List Archives
>http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
>List Instructions (How to leave etc.)
>http://www.jiscmail.ac.uk/
>
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual who posts and they are solely responsible for all message content. The ACB does not monitor posts.
ACB Web Site
http://www.acb.org.uk
Green Laboratories Work
http://www.laboratorymedicine.nhs.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
|