Hi,
> when doing particle alignment in 3D in Relion, does the expectation step
> take into account previous information from 2D classification?
No.
Best regards,
Takanori Nakane
> Dear Relion community,
>
> when doing particle alignment in 3D in Relion, does the expectation step
> take into account previous information from 2D classification?
> E.g. if 10,000 particles went into a single class in 2D, the likelihood
> for these 10,000 particles to be in a more or less similar orientation in
> 3D should be high, right?
> At least the psi angle for each of these particles should be the same /
> change the same way.
>
> Thank you for helping me understand better.
> If the answer to this question is yes, then it would always be useful to
> do a 2D classification before starting a 3D classification, I guess.
>
> Best,
> Thomas
>
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