Dear Shirin,
Focused classification with partial signal subtraction might do the trick.
See the paper in eLife on sampling the conformational landscape of
gamma-secretase in eLife (Bai et al).
HTH,
Sjors
> Hello everyone,
>
> Is there a way to classify particles (ribosome-protein complex) on basis
> of
> presence or absence of a protein density of ~ 60kd (present on the surface
> and clearly visible) in relion? Masked 3d classification could identify
> some conformational changes in protein interaction but i am not being able
> to isolate a class where the protein is not bound at all.
> Any tricks or suggestions?
>
> thanks,
> Shirin
>
>
> --
> *Shirin *
>
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Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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