Hi,
Did you try Sjors' suggestion earlier today?
> I did motioncorr in Relion 3.0 and the particle file is from Warp
Probably the rlnMicrographName column in your particle STAR file
points to somewhere else. This should point to micrographs under
MotionCorr/job296/.
Best regards,
Takanori Nakane
On 2019/04/03 20:32, Da Cui wrote:
> Dear community,
> I have a question about Bayesian polishing. When I run Bayesian polishing, I always got the error message as below:
> in: /programs/x86_64-linux/relion/3.0_cu8.0/src/jaz/micrograph_handler.cpp, line 529
> === Backtrace ===
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN11RelionErrorC1ERKSsS1_l+0x41) [0x426b71]
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN17MicrographHandler11getMetaNameESs+0x1b5) [0x4994c5]
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN17MicrographHandler11loadInitialERK13MetaDataTabledbRiRdRSs+0x109) [0x49bb69]
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN17MicrographHandler4initERK13MetaDataTabledbiRiRdRSs+0x7f) [0x49cf8f]
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN13MotionRefiner4initEv+0x4c3) [0x4564d3]
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(main+0x31) [0x423591]
> /lib64/libc.so.6(__libc_start_main+0xf5) [0x2aaab9b45af5]
> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine() [0x4240e1]
> ==================
> ERROR:
> ERROR: MicrographHandler::getMetaName: no metadata star-file for 17nov06b_00005gr_00006sq_v02_00002hln_v01_00002enn.frames.tif found in MotionCorr/job296/corrected_micrographs.star.
>
> However, I checked the path of each file, and I found nothing wrong (every file is there). I did motioncorr in Relion 3.0 and the particle file is from Warp, but refined and postprocessed in Relion. Can you please provide any solution to that problem? Really hope polishing can improve my resolution even further:)
> Thank you so much in advance.
>
> Best wishes,
> Da
>
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