Hi,
> Polishing is working fine now, although it seems to take forever to
write our the particle star
> file. Is it normal?
This depends on your box size, CPU, system memory, number of particles,
movies, frames, MPIs and threads.
Best regards,
Takanori Nakane
> ---Da
> ________________________________
> From: Takanori Nakane <[log in to unmask]>
> Sent: Wednesday, April 3, 2019 5:47 PM
> To: Da Cui; [log in to unmask]
> Subject: Re: [ccpem] Error no metadata star file during Bayesian polishing
>
> Hi,
>
> Did you try Sjors' suggestion earlier today?
>
> > I did motioncorr in Relion 3.0 and the particle file is from Warp
>
> Probably the rlnMicrographName column in your particle STAR file
> points to somewhere else. This should point to micrographs under
> MotionCorr/job296/.
>
> Best regards,
>
> Takanori Nakane
>
> On 2019/04/03 20:32, Da Cui wrote:
>> Dear community,
>> I have a question about Bayesian polishing. When I run Bayesian
>> polishing, I always got the error message as below:
>> in:
>> /programs/x86_64-linux/relion/3.0_cu8.0/src/jaz/micrograph_handler.cpp,
>> line 529
>> === Backtrace ===
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN11RelionErrorC1ERKSsS1_l+0x41)
>> [0x426b71]
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN17MicrographHandler11getMetaNameESs+0x1b5)
>> [0x4994c5]
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN17MicrographHandler11loadInitialERK13MetaDataTabledbRiRdRSs+0x109)
>> [0x49bb69]
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN17MicrographHandler4initERK13MetaDataTabledbiRiRdRSs+0x7f)
>> [0x49cf8f]
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(_ZN13MotionRefiner4initEv+0x4c3)
>> [0x4564d3]
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine(main+0x31)
>> [0x423591]
>> /lib64/libc.so.6(__libc_start_main+0xf5) [0x2aaab9b45af5]
>> /programs/x86_64-linux/relion/3.0_cu8.0/bin/relion_motion_refine()
>> [0x4240e1]
>> ==================
>> ERROR:
>> ERROR: MicrographHandler::getMetaName: no metadata star-file for
>> 17nov06b_00005gr_00006sq_v02_00002hln_v01_00002enn.frames.tif found in
>> MotionCorr/job296/corrected_micrographs.star.
>>
>> However, I checked the path of each file, and I found nothing wrong
>> (every file is there). I did motioncorr in Relion 3.0 and the
>> particle file is from Warp, but refined and postprocessed in Relion.
>> Can you please provide any solution to that problem? Really hope
>> polishing can improve my resolution even further:)
>> Thank you so much in advance.
>>
>> Best wishes,
>> Da
>>
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