Hello everyone,
I have recently installed relion3.0_beta. This has allowed my to get my resolution from 4.4 A to 3.7 A, which is at the Nyquist frequency for my downscaled dataset (1.1 A pixel size, particle boxes downscaled from 500 to 300 pixels, 320 A particle diameter). To get the best possible resolution I have now reextracet the same downscaled particles to full 1.1 A pixel size, redone 3D classification, 3D refinement, mask creastion and post processing. Now, trying to do the CTF refinement on this dataset, I get the following error though:
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terminate called after throwing an instance of 'std::bad_alloc'
what(): std::bad_alloc
[bamf:23014] *** Process received signal ***
[bamf:23014] Signal: Aborted (6)
[bamf:23014] Signal code: (-6)
[bamf:23014] [ 0] /lib/x86_64-linux-gnu/libpthread.so.0(+0x11390)[0x7f736ca20390]
[bamf:23014] [ 1] /lib/x86_64-linux-gnu/libc.so.6(gsignal+0x38)[0x7f736bc02428]
[bamf:23014] [ 2] /lib/x86_64-linux-gnu/libc.so.6(abort+0x16a)[0x7f736bc0402a]
[bamf:23014] [ 3] /home/hilvertg/anaconda2/lib/libstdc++.so.6(_ZN9__gnu_cxx27__verbose_terminate_handlerEv+0xbc)[0x7f736c76ab39]
[bamf:23014] [ 4] /home/hilvertg/anaconda2/lib/libstdc++.so.6(+0x941fb)[0x7f736c7691fb]
[bamf:23014] [ 5] /home/hilvertg/anaconda2/lib/libstdc++.so.6(+0x94234)[0x7f736c769234]
[bamf:23014] [ 6] /home/hilvertg/anaconda2/lib/libstdc++.so.6(__cxa_rethrow+0x0)[0x7f736c769416]
[bamf:23014] [ 7] /home/hilvertg/anaconda2/lib/libstdc++.so.6(_Znwm+0x53)[0x7f736c7697b4]
[bamf:23014] [ 8] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(_ZN13MultidimArrayI8tComplexIdEE12coreAllocateEv+0x56)[0x499f86]
[bamf:23014] [ 9] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(_ZN18FourierTransformer7setRealER13MultidimArrayIdE+0x62)[0x531702]
[bamf:23014] [10] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(_ZN9Projector26computeFourierTransformMapER13MultidimArrayIdES2_iibb+0x1be2)[0x512f02]
[bamf:23014] [11] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(_ZN12ReferenceMap4loadEib+0x925)[0x4f3765]
[bamf:23014] [12] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(_ZN10CtfRefiner4initEv+0xd5f)[0x47d81f]
[bamf:23014] [13] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(main+0x44)[0x4263a4]
[bamf:23014] [14] /lib/x86_64-linux-gnu/libc.so.6(__libc_start_main+0xf0)[0x7f736bbed830]
[bamf:23014] [15] /home/hilvertg/relion-3.0_beta/build/bin/relion_ctf_refine_mpi(_start+0x29)[0x426e79]
[bamf:23014] *** End of error message ***
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Any ideas?
Stephan
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