Hi all,
I got this message when I was using relion to do auto-refine, here is my command and the error message:
COMMAND:
`which relion_refine` --o Refine3D/job009/run --auto_refine --i selected.star --ref InitialModel/10iter/run_it009_class001.mrc --firstiter_cc --ini_high 60 --dont_combine_weights_via_disc --pool 3 --particle_diameter 70 --flatten_solvent --zero_mask --oversampling 1 --healpix_order 2 --auto_local_healpix_order 4 --offset_range 5 --offset_step 2 --sym C3 --low_resol_join_halves 40 --norm --scale --j 13 --angpix 2.7 --dont_check_norm
ERROR MESSAGE:
Auto-refine: Estimated accuracy angles= 30.1 degrees; offsets= 2.185 pixels
Auto-refine: WARNING: The angular accuracy is worse than 10 degrees, so basically you cannot align your particles (yet)!
Auto-refine: WARNING: You probably need not worry if the accuracy improves during the next few iterations.
Auto-refine: WARNING: However, if the problem persists it may lead to spurious FSC curves, so be wary of inflated resolution estimates...
Auto-refine: WARNING: Sometimes it is better to tune resolution yourself by adjusting T in a 3D-classification with a single class.
ERROR:
MlOptimiser::updateAngularSampling: BUG! updating of angular sampling should only happen for gold-standard (auto-) refinements.
File: /sandbox/home/relion/relion/src/ml_optimiser.cpp line: 7825
Thank you all in advance for your time. I really appreciate your help.
Best,
WL
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