Hi Sjors and CCPEM members,
I have about one thousand movie-stack (50 frames each) micrographs, and the artifacts are obvious after gain correction (vertical lines in FFT of aligned micrographs).
You mentioned a posteriori method to correct gain of micrographs and I would like to give it a try. I am wondering if Relion 2.0 can do such operation. can you point me the commands I should use?
Thank you very much for your help!
xiaodong
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