Dear all,
When I perform 3D auto-refine of helical specimen with solvent-flattening FSC mode, I encounter the following error
Expectation iteration 1
25.77/25.77 min ............................................................~~(,_,">
Applying helical symmetry from the last iteration in real space...
Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ...
Note that only for higher resolutions the FSC-values are according to the gold-standard!
Calculating solvent-corrected gold-standard FSC ...
+ randomize phases beyond: -345.6 Angstroms
Postprocessing::run ERROR: FSC curve never drops below randomize_fsc_at. You may want to check your mask.
File: /home/people/tanaka/tanaka602/relion2-beta-20161128/src/ml_optimiser_mpi.cpp line: 2261
Of course I checked the mask and there seems no strange in it. Postprocessing of the previous refinement with the same mask produces a nomal map and healthy FSC curve. I tried several different masks, different version of relion2-beta and relion2-stable, but the situation was the same.
I think this error is the same as the previous post by Dieter Blaas "Relion2: ERROR: FSC curve never drops below randomize_fsc_at. You may want to check your mask."
I appreciate any suggestion. Thank you in advance.
Best Regards,
Kotaro Tanaka
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