Dear Sjors and Jiang,
Thank you much for your comments and suggestions!
I'll try --incr_size 15 first.
Best Regards,
Kotaro Tanaka
> -----Original Message-----
> From: Sjors Scheres [mailto:[log in to unmask]]
> Sent: Thursday, March 02, 2017 1:10 AM
> To: Kotaro Tanaka <[log in to unmask]>;
> [log in to unmask]
> Subject: Re: [ccpem] Sovent flattening FSC error in helical refinement
>
> Hi Kotaro,
> We've also encountered this ourselves. It will be fixed in a future version. For
> now, you could try to use the same parameters as before, but add the
> following to the additional arguments: --incr_size 15 By default, one takes 10
> Fourier shells beyond the initial resolution estimate into account (--incr_size
> 10). In your case, the unmasked FSCs still correlate more than 0.8 within that
> range. By increasing the range, they should drop below and all should be well.
> HTH,
> Sjors
>
>
> On 03/01/2017 04:17 AM, Kotaro Tanaka wrote:
> > Dear all,
> >
> > I noticed that the unmasked fsc of the two maps was too high in my case.
> >
> > The minimum of the unmasked FSC was 0.81, which is higher than the
> determination threshold of 0.8 for phase-randomization start resolution.
> >
> > I will try to lower the unmasked FSC in the very early iterations. Maybe by
> reducing Initial lowpass-filter value (now 20 A).
> >
> > Thank you.
> >
> > Best Regards,
> > Kotaro Tanaka
>
> --
> Sjors Scheres
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH,
> U.K.
> tel: +44 (0)1223 267061
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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