Elena, if you are modifying your 2x binned STAR file, then make sure you're also updating the _rlnDetectorPixelSize column, i.e. from 10um (2x bin) to 5um (unbinned) for K2 data.
Peter
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From: Collaborative Computational Project in Electron cryo-Microscopy [[log in to unmask]] on behalf of Elena Zehr [[log in to unmask]]
Sent: Thursday, October 06, 2016 7:08 AM
To: [log in to unmask]
Subject: Re: [ccpem] Drop in resolution going from 2x binned to 1x data
Hi Sjors and everyone,
Thank you very much for your feedback.
I put no for "map on gray scale" and changed pixel size
I think maybe my map is not scaled correctly. I use the following command:
relion_image_handler --i map_2x.mrc --new_box (old box size *2) --rescale_angpix (old pixel size /2) --o map_1X.mrc
Is this the right command to scale the map or I also need something else.
Thank you so much again!
Elena
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