Hi folks,
I did not see any posts about this issue on this forum, so I need your advice.
I unbinned data from 2x to 1x, modified star file (detector pixel size, origins x and y) and scaled the reference volume (as was discussed previously here). Then, I refined the reconstruction with 1x data, but after the refinement (3D auto refine option) the resolution went from 8A (2x binned data) to 20A (1x binned data).
Same is true when I attempt to perform 3D classification with global image alignment or local angular searches, with the resolution steadily decreasing after each reconstruction round.
Has anyone experienced something similar?
Thanks,
Elena
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