When I use the ‘Optimize B’ in 'Map Sharpening...', Coot chooses to blur my 2Fo-Fc map to the maximum possible extent (+200). Can anyone explain this? I was rather hoping to get a sharpened map… I can adjust the sharpening manually, but I wonder why Coot chooses to blur it. The map is from the mtz file produced in Phenix Refine. It’s at 3.9A and with high NCS, Coot is 0.8.3.
Thanks for any help.
Luca
Luca Pellegrini, PhD
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Cambridge CB2 1GA
UK
Email: [log in to unmask]
Phone: 0044-1223-760469
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