The fumes are a bit scary really. I can recall at CXH scintillation counting using Toluene and a weird fluor called pop op (I have almost certainly got this wrong so please correct) I think it was a phosphor based compound. As a minion in the dept, I can again recall washing the reaction vials in very hot water, the smell of aromatic toluene lives with me always, the fact it is now recognised as a carcinogen passed under the health and safety radar in 1978. The obsession with getting radiation out of routine lab testing followed Chernobyl in 1986. I was always a bit concerned about the social programme at ACB Focus that year in Blackpool (May ?), climbing some minor peak in the Lakes, in lots of rain and Iodine and Strontium fallout. Being a biker at the time, I had Barbour gear, so impenetrable and totally safe.
I can also recall that the use of Iodine isotopes in anything vaguely Thyroid testing related caused sensational interest, totally disproportionate to the danger . Other minor exposure to radiation included radioactive phosphorous labelling on the 6th floor for the progesterone assay, plus oodles of ether for the 17 oxo steroid used in the Zimmermann reaction (nothing to do with Bob Dylan) . I can also recall the delightful Mattingly method for Cortisol analysis a sweet concoction methylene chloride and sulphuric acid. This was a great trouser dissolver. Far more dangerous was the agitation between adept and master in the dept as to the alignment/angle of the detector to pick up the transmitted light.
I have come full circle ending up at the RDE where Mattingly developed the method.
Thanks Jan for the reminder, I have lost many garments in this trade over the decades, some through corrosion, others neglect.
Bless you all
John
________________________________________
From: Clinical biochemistry discussion list [[log in to unmask]] On Behalf Of jan still [[log in to unmask]]
Sent: 27 January 2016 18:33
To: [log in to unmask]
Subject: Re: Early days
For fumes and holes in clothing and shoes you couldn't beat the protein bound iodine digester! Acetic anhydride, fuming nitric and conc sulfuric certainly did a good job. It's a wonder any of us survived.
Kind regards
Jan Still
Sent from Samsung Mobile
-------- Original message --------
From: "Bosomworth Mike (LEEDS TEACHING HOSPITALS NHS TRUST)" <[log in to unmask]>
Date: 27/01/2016 18:10 (GMT+00:00)
To: [log in to unmask]
Subject: Re: Early days
And burnt through denim in no time - not that I ever wore jeans for work!
Dr Mike Bosomworth
Clinical Service Lead for Blood Sciences and Specialist Laboratory Medicine
Tel: 0113 3922340
Mobile: 07789174344
In April - May I cycled solo from New York to San Francisco (4,320 miles), entirely self-funded, to raise money for the Yorkshire Brain Research Centre. If you would like to sponsor me then please visit:
www.virginmoneygiving.com/mikebosomworth. For more information about the ride and the charity please visit:
http://www.ybrc.org.uk/dr-mike-back-on-the-bike/
If you have already sponsored me then many thanks
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Waller, Paul
Sent: 23 January 2016 11:22
To: [log in to unmask]
Subject: Re: Early days
How embarrassing for someone in my job - a real student's error; my ex- Head of Department (Dennis Wright) reminds me that the Kober-Ittrich reaction uses sulphuric acid, not HCl.
Still produced a lot of fumes though!
Paul Waller MSc CSci FIBMS FHEA
Associate Professor in Biomedical Science Head, Biomolecular Sciences Department School of Life Sciences, Pharmacy and Chemistry T 020 8417 7783 (Direct Dial) / 67783 (Internal) E [log in to unmask] / Web page Mr Paul Waller
Room PR MB 1071, Penrhyn Road, Kingston upon Thames, KT1 2EE Note new room number!
Office hours 1:00-2:30 Tuesdays and Fridays
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Waller, Paul
Sent: 23 January 2016 10:42
To: [log in to unmask]
Subject: Re: Early days
I was at Charing Cross at the same time as John, and did my FIMLS project on curve regeneration.
Key lesson; don't use tissues to mop up the HCl when the oestriol analyser springs a leak - the fumes will set the fire alarm off.
Paul Waller MSc CSci FIBMS FHEA
Associate Professor in Biomedical Science Head, Biomolecular Sciences Department School of Life Sciences, Pharmacy and Chemistry T 020 8417 7783 (Direct Dial) / 67783 (Internal) E [log in to unmask] / Web page Mr Paul Waller
Room PR MB 1071, Penrhyn Road, Kingston upon Thames, KT1 2EE Note new room number!
Office hours 1:00-2:30 Tuesdays and Fridays
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of OConnor John (ROYAL DEVON AND EXETER NHS FOUNDATION TRUST)
Sent: 23 January 2016 08:48
To: [log in to unmask]
Subject: Re: Early days
Plenty of experience at Charing X. AAI for uriniary oestriol, sampler looked like nodding donkey, water sample between specimen to reduce carry over and the delights of Kober reagent and peristaltic pumps. AA2 continuous flow analysis for just about most other things. Remember using embroidery hoops to stretch the semi permeable membranes over the dialyser disks. Also the Technicon 6/60 was supercharged to 6/120 using the famed O'Donovan box and curve regeneration, drift control every 10 samples and physically tweaking the timer knobs to synchronise the flow. Plus one's own personal library of timing coils.
A day on the 12 channel beast, trying to keep up with manually reading the data using a graticule, was real hard work !!
All praise to Skeggs, was a lot more fun in those days.
BW John
________________________________________
From: Clinical biochemistry discussion list [[log in to unmask]] On Behalf Of Mike Addison [[log in to unmask]]
Sent: 22 January 2016 19:01
To: [log in to unmask]
Subject: Early days
I do not think that any active member of the ACB has direct experience of the Technicon AA1 or AAII analysers but if you do or know of someone, maybe retired, who has please contact me.
Mike Addison
[log in to unmask]
Sent from my iPad
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Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
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