Hi Ian,
I'm not sure which tutorial you are following. Please download the pdf
from the RELION wiki:
http://www2.mrc-lmb.cam.ac.uk/groups/scheres/relion13_tutorial.pdf
It should be about betagal, not ribo. And the command to copy the
precalculated manual picks should be:
cp PrecalculatedResults/Micrographs/*_manual.star Mic
rographs/.
HTH,
Sjors
> Hello, I'm working through the Relion 1.3 tutorial material, using the
> download with the ribo images, & I got as far as section 2.3 'Manual
> particle picking'. I picked a few manually & decided that I had got the
> general idea (I know, chicken!) and went for the easy option to copy the
> precalculated ones. However the command (from the 'ribo' directory):
>
> cp ../PrecalculatedResults/Micrographs/*_manual.star Micrographs
>
> just gives:
>
> cp: No match.
>
> and indeed there are no files matching that pattern. Also they are not in
> the relion_tutorial.tar.gz file that I originally downloaded. I
> downloaded
> it again in case it had been updated since I downloaded it, but still no
> luck. There are however .box files for each image in that directory. Is
> there somewhere I can pick up the missing .star files or should I just use
> the .box files (and if so, how)?
>
> Cheers
>
> -- Ian Tickle (Astex)
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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