Dear Chuanglu,
If the reconstruction comes from RELION, then the absolute intensity scale
of the reconstructions should be the same as the signal in the
experimental particles, and the subtraction should go OK. You should use
the same CTF options as in the 3D refinement, so if for example you
ignored CTFs until the first peak in refinement, you should also do so in
the subtraction.
HTH,
Sjors
> Dear Sjors and list
>
> I was running relion_projec as described in paper "bioRxiv" with software
> Relion1.4beta. The problem is that projections generated from "yellow.mrc"
> have much higher signal/noise ratio and higher pixel value than
> experimental data. When I subtract projection of "yellow.mrc" from
> experimental image, the remaining data is almost same as projection data.
>
>
> So how the projection data should be modified or added with noise that it
> could have similar pixel value as experimental data?
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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