Hi Leo,
Are the final results of your short-cut run still OK? If so, I wouldn't
worry about the numbers there.
S
On 09/11/2015 02:39 PM, Leonid Sazanov wrote:
> Hi,
>
> To save computing time, we are trying to start 3D autorefine in local mode, i.e. using .star file where particle orientations are close to final, so we just want to do local refinement. Things seem to work OK, however, I noticed that if we set initial sampling to 1.8 degrees, keeping starting of local refinement at 1.8, initially for several iterations (until sampling rate increases) we see the following:
>
> Oversampling= 0 NrHiddenVariableSamplingPoints= 9
> OrientationalSampling= 3.75 NrOrientations= 1
> TranslationalSampling= 2 NrTranslations= 9
> =============================
> Oversampling= 1 NrHiddenVariableSamplingPoints= 288
> OrientationalSampling= 1.875 NrOrientations= 8
> TranslationalSampling= 1 NrTranslations= 36
>
>
> On the other hand, if 3D autorefine reaches similar point in a usual way, starting from 7.5 degree sampling, then for OrientationalSampling= 1.875 NrOrientations will be 145, not 8.
>
> Not clear to me why the difference?
> Many thanks!
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|