Dear All,
I found a strong correlation between lipaemic index and triglyceride concentration measured on a Roche COBAS 8000 analyser, in a very small experiment using samples spiked with 20% intralipid. See attached chart.
Best wishes,
Paul
Specialty Registrar in Chemical Pathology (Metabolic Medicine)
Belfast Health and Social Care Trust
Department of Clinical Biochemistry, Kelvin Building, Royal Victoria Hospital
Grosvenor Road, Belfast, Northern Ireland, BT12 6BA
--------------------------------------------
On Wed, 2/9/15, Waller, Paul <[log in to unmask]> wrote:
Subject: Re: Lipaemia index
To: [log in to unmask]
Date: Wednesday, 2 September, 2015, 15:34
To add my own unpublished
observations;
Intralipid
behaves very differently to endogenous chylomicrons, and can
cause apparent interference in kinetic assays. My unproven
hypothesis was that this was due to detergents/surfactants
in reagents breaking down the lipid vesicles and thus
causing a decrease in absorbance due to turbidity.
I did not see this phenomenon
to the same extent in lipaemic patients sera.
Paul Waller
MSc CSci FIBMS FHEA
Associate Professor in
Biomedical Science
Associate Head
Biomolecular Sciences
School of Life
Sciences, Pharmacy and Chemistry
T 020 8417
7783 (Direct Dial) / 67783 (Internal)
E [log in to unmask]
/ Web page Mr Paul Waller
Room PR MB 1019, Penrhyn Road, Kingston upon
Thames, KT1 2EE
Office
hours by appointment
-----Original Message-----
From: Clinical biochemistry discussion list
[mailto:[log in to unmask]]
On Behalf Of [log in to unmask]
Sent: 02 September 2015 15:24
To: [log in to unmask]
Subject: Re: Lipaemia index
Well put, Graham. I was going to reply
similarly, but I think you describe very well the
variability in chylomicron particle size and their
triglyceride content. As I mentioned previously in this
thread, several publications and my own unpublished
observations show that there is very little correlation
between L (or T)-index and triglyceride concentration in
lipemic specimens.
Note
that Soha Zouwail mentioned to me off list that she found
good correlation between the triglyceride concentration in
Intralipid and L-index. I have used Intralipid to check for
lipemia (turbidity) interference, but did not look at the
correlation with the L-index. In fact, I think the last time
I did such interference studies was before the specimen
indices era. I think this makes sense though because the
lipid vesicles of Intralipid are likely to be much more
uniform than chylomicrons.
-Jim
-----Original Message-----
From: Clinical biochemistry discussion list
[mailto:[log in to unmask]]
On Behalf Of Graham Jones
Sent: Wednesday,
September 02, 2015 8:27 AM
To: [log in to unmask]
Subject: Re: Lipaemia index
Derreck,
I
expect not. Rather than having a lot of nice fresh
chylomicrons, all the same size and same triglycerides (TG)
content (and perhaps the same light scattering), I would
expect in any sample we may have a range of difference sizes
and TG content (and light scattering) as they lose TG to
tissues on their way to the liver. Thus the relationship
between TG and light scattering is not very close.
Just my thoughts,
Graham
________________________________________
From: Clinical biochemistry discussion list
<[log in to unmask]>
on behalf of Mccullough Derreck (UHMB) <[log in to unmask]>
Sent: Wednesday, 2 September 2015 6:45 PM
To: [log in to unmask]
Subject: Re: Lipaemia index
Hi Jim,
As chylomicrons are
90% triglyceride would you not expect a good correlation
between triglycerides and turbidity if turbidity is caused
by chylomicrons?
Bw,
Derreck
-----Original Message-----
From: Clinical biochemistry discussion list
[mailto:[log in to unmask]]
On Behalf Of [log in to unmask]
Sent: 26 August 2015 18:14
To:
[log in to unmask]
Subject: Re: Lipaemia index
Replying to myself here! I agree with Alan that
Sonia's triglyceride vs L- (or T-index) is much higher
than expected. I've had a chance to dig out one of my
correlations: Triglyceride, mg/dL = 0.88 L-index + 185, R^2
= 0.017. (Sorry for U.S. units, but the key here is the very
low R^2.) My bad that I didn't indicate the
instrument/method on the graph, but it was a Roche
instrument, probably an Integra. Also, earlier I implied
that VLDL, being triglyceride rich, would increase the
L/T-index. VLDL rarely does, but most often it is
chylomicrons that cause the turbidity.
Sonia, you mention that your L-index results
are binned, that is, 1+, 2+, etc. yet your graph has numbers
for the L-index. Does your Architect give you both the
numerical L-index and the binned result?
-Jim
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