Dear Giulliana Rangel,
if your protein is made recombinantly you could try a SeMet prep. The
fixed atoms solve many issues you could have with soaking.
Best,
Tim
On 06/29/2015 02:47 PM, Giulliana Rangel wrote:
> Dear all,
>
> I'm looking for a method to solve the phase problem. Thus, I would like
> some help about heavy-atom derivatives.
>
> What is the best heavy-atom to make soaking? I tried Iodine (because my
> protein have a lot of tyrosines), but no results...
>
> I read some papers about quick-soak, its a good method? Using platine, Hg,
> Br...
>
> Any suggestions? Thank you so much.
>
> Best wishes,
>
--
--
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
phone: +49 (0)551 39 22149
GPG Key ID = A46BEE1A
|