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Dear Giulliana Rangel, if your protein is made recombinantly you could try a SeMet prep. The fixed atoms solve many issues you could have with soaking. Best, Tim On 06/29/2015 02:47 PM, Giulliana Rangel wrote: > Dear all, > > I'm looking for a method to solve the phase problem. Thus, I would like > some help about heavy-atom derivatives. > > What is the best heavy-atom to make soaking? I tried Iodine (because my > protein have a lot of tyrosines), but no results... > > I read some papers about quick-soak, its a good method? Using platine, Hg, > Br... > > Any suggestions? Thank you so much. > > Best wishes, > -- -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A