I pretty sure that it is. I followed the tutorial and everything was
looking good. Then when I had the problem the first thing I tried was
using the STAR file from the PrecalculatedResults folder to see if I had
messed something else up along the way, but had the same issue. Thanks for
the help.
Bryan
On 11/19/14, 1:31 AM, "Sjors Scheres" <[log in to unmask]> wrote:
>Hi Bryan,
>The autopicking for the tutorial should only take ~0.5Gb of RAM, so that's
>not the problem. Are you sure the input STAR file with the 2 micrographs
>and their CTF information is correct?
>HTH,
>S
>
>> Hi,
>>
>> I'm new to using RELION so I was running through the tutorial data and
>> associated documentation. My problem is I'm on the step where you
>>perform
>> the Auto-picking on 2 micrographs and create the FOM maps. In the
>>tutorial
>> it takes 4-5 min per micrograph on their computers. However, when I
>>click
>> run on mine I can see the command start if I have htop running, but it
>> only is active for 30 seconds or so then seems to end. I don't see any
>> errors pop up in the terminal used to start the RELION user interface,
>>but
>> when I go to look at the micrographs in the Micrograph inspection step
>>no
>> particles have been picked. I do get an error however if I try to move
>>on
>> in the tutorial and switch the auto-picking steps from Write FOM maps to
>> Read FOM maps. The error is suggesting a memory issue, but this
>>computer
>> should have plenty of memory (64 GB of RAM). Any suggestions/tips would
>> be greatly appreciated. Thanks in advance for the help
>>
>> Bryan Hansen
>> Electron Micrscopist
>> NIH/NIAID/RTB/RML/EMU
>> Rocky Mountain Laboratories
>> 903 S. 4th St
>> Hamilton, MT 59840
>> 406.363.9202<tel:406.363.9202>
>>
>
>
>--
>Sjors Scheres
>MRC Laboratory of Molecular Biology
>Francis Crick Avenue, Cambridge Biomedical Campus
>Cambridge CB2 0QH, U.K.
>tel: +44 (0)1223 267061
>http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>
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