Hi Bryan,
The autopicking for the tutorial should only take ~0.5Gb of RAM, so that's
not the problem. Are you sure the input STAR file with the 2 micrographs
and their CTF information is correct?
HTH,
S
> Hi,
>
> I'm new to using RELION so I was running through the tutorial data and
> associated documentation. My problem is I'm on the step where you perform
> the Auto-picking on 2 micrographs and create the FOM maps. In the tutorial
> it takes 4-5 min per micrograph on their computers. However, when I click
> run on mine I can see the command start if I have htop running, but it
> only is active for 30 seconds or so then seems to end. I don't see any
> errors pop up in the terminal used to start the RELION user interface, but
> when I go to look at the micrographs in the Micrograph inspection step no
> particles have been picked. I do get an error however if I try to move on
> in the tutorial and switch the auto-picking steps from Write FOM maps to
> Read FOM maps. The error is suggesting a memory issue, but this computer
> should have plenty of memory (64 GB of RAM). Any suggestions/tips would
> be greatly appreciated. Thanks in advance for the help
>
> Bryan Hansen
> Electron Micrscopist
> NIH/NIAID/RTB/RML/EMU
> Rocky Mountain Laboratories
> 903 S. 4th St
> Hamilton, MT 59840
> 406.363.9202<tel:406.363.9202>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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