Hi,
I think the experiment is doable, but how would you decouple
protein-protein interaction from folding of the unfolded
protein due to protein interaction?
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
---- Original message ----
>Date: Fri, 14 Mar 2014 18:07:48 +0530
>From: CCP4 bulletin board <[log in to unmask]> (on behalf
of Anita P <[log in to unmask]>)
>Subject: [ccp4bb] ITC with unfolded proteins
>To: [log in to unmask]
>
> Hello everyone,
> I have a query for the scientists working on
> protein-protein interaction.
> It is known that some proteins exist in unfolded or
> molten globule state and attain structure on
> interaction with other folded proteins.
> Many a times, it is difficult to obtain the
> structure of these complexes.
> Is it possible to quantitatively determine the
> thermodynamics of interaction between an unfolded
> protein and a folded protein using ITC? Later may be
> perform an alascan to determine the residues of the
> unfolded partner involved in the interaction.
> Please share your ideas
> cheers**
> Anita
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