Le Vendredi 14 Mars 2014 13:37 CET, Anita P <[log in to unmask]> a écrit:
Anita,
If one of the partners is indeed more or less unfolded before interaction, then you should see a negative DeltaS upon complex formation.
Practically, I would try first putting the unfolded protein in the cell and the folded one in the syringe in view of possible solubility problems with an "incorrectly" folded protein.
Alanine scanning ? Yes on the paper, but may be less feasible in practice due to the amount of material to prepare.
Good luck
Philippe Dumas
> Hello everyone,
>
> I have a query for the scientists working on protein-protein interaction.
> It is known that some proteins exist in unfolded or molten globule state
> and attain structure on interaction with other folded proteins.
> Many a times, it is difficult to obtain the structure of these complexes.
>
> Is it possible to quantitatively determine the thermodynamics of
> interaction between an unfolded protein and a folded protein using ITC?
> Later may be perform an alascan to determine the residues of the unfolded
> partner involved in the interaction.
>
> Please share your ideas
>
> cheers
> Anita
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