Hi Piotr,
with regard to your Aria question:
One of our students got this error message a few weeks ago and I think
it turned out that the aliased region was incorrect. You need to go to
'Spectrum' in the 'Experiments' menu and then the 'Referencing' tab.
Check for each of your Noesy spectra that the minimum and maximum
aliased frequencies are correct. I think in our case there had been a
problem with the C dimension in the aromatic Noesy. I guess Aria only
tries to make assignments within the aliased region of a spectrum or
something like that. I'm not quite sure how we can have ended up with
peaks in an unaliased part of the spectrum, or perhaps the problem was
that the peaks were also at the wrong carbon positions. But anyway, I
think the problem was basically down to some sort of aliasing issue of
this kind. That may be what Aria means with "undersized frequency windows".
Vicky
On 19/06/13 14:27, Piotr Wysoczanski wrote:
> Hi again,
>
> I have 2 questions. One related to CYANA and one to ARIA, but in strict relation to ANALYSIS.
>
> I tried recently to compare performance of the 2 above on my recent project and I get this message in ARIA:
>
> WARNING [Project]: More than 90% of the cross peaks could not be assigned. This
> might be due to interchanged frequency dimensions or
> undersized frequency windows. Please check your setup.
>
> Now the same dataset I manged to successfully export and use for CYANA, also I don't have any "red" assignments related to wrong dimension mapping, but my spectra are sometimes processed by NMRpipe (I swap the regular bruker dimension order) and sometimes by topspin, so in some cases the dimension order is indeed different, but as I wrote ANALYSIS does not have problems with this.
>
> The second question. Is there any quick way to import peaklists from CYANA? I have a set of 9 NOESY spectra and doing that one by one through format converter takes quite a bit of time.
>
> All the best,
>
> Piotr
--
************************************************
Dr. Victoria A. Higman-Davies
School of Chemistry
University of Bristol
Cantock's Close
Bristol BS8 1TS
U.K.
E-mail: [log in to unmask]
http://www.protein-nmr.org.uk
************************************************
|