Dear RELION users,
As the grouping of micrographs has raised many questions lately and
because small groups can lead to errors during refinement, I have now
implemented a new micrograph grouping procedure. As of
relion-1.2-beta-10, you may use the label rlnGroupName in your
particle.star file to group different micrographs together. To further
facilitate this process, I have also written an auxiliary script. For
more info see the FAQs on the RELION wiki.
As always, if you want the latest beta-version, just email me. Hopefully
the official 1.2 release will be made soon....
have fun,
Sjors
On 03/25/2013 04:11 PM, Brunner,Matthias wrote:
> Hi,
>
> thanks for the helpful suggestions in response to my previous mail!
> I have another one:
>
> Following Scenario:
>
> I classified my dataset into 10 3D classes and now want to auto-refine one of the classes.
> I extracted a class into a new STAR file (~15k particles)...
>
> Of course the particle number is now greatly reduced and therefore I get a lot of warnings of the kind:
> "WARNING: There are only 2 particles in group 2016 of half-set 1"
>
>
>
>
> How should I handle this? Just turn off the intensity scale correction?
> Or is there a way to tell relion to re-use the more reliable intensity scale corrections that were previously determined from the larger, aggregate dataset?
> And should this be done?
>
> In general, what are the suggested options to auto-refine a 3D class. Are they in any way different from the first auto-refine step before 3D classification?
>
>
> Cheers
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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