You can get reagent carryover from the ICT diluent unless you use the smartwash: R1/ICTD5/0.5%acid wash.
Mike Collins
BMS3
Biochemistry Automation
Norfolk & Norwich University Hospital
England
[log in to unmask]
http://www.nnuh.nhs.uk/
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of mpeake
Sent: 23 February 2013 00:00
To: [log in to unmask]
Subject: Re: Abbott users - serum calcium
Maria,
We have also had calcium calibration problems in the past with reagents from
a different company.
One way to investigate this problem is as follows:
1. Ask Abbott to help you solve this problem by purchasing some NIST 956C
(Electrolytes in frozen human serum, see attachment).
2. This Standard Reference Material provides sera with three calcium
concentrations covering the range 2.1 -> 3.0 mmol/L.
3. After carefully calibrating your assay, run these sera in triplicate (
also assay your calibrators in triplicate at the same time to prove your
assay is calibrated correctly ).
4. If you (or Abbott) know someone with a higher order calcium assay, ask
them to assay some patient samples for you in duplicate ( include SRM 956C
in both assays).
5. Agree to share all results with Abbott in case their calibration values
need adjusting.
6. It would also be useful to ask a colleague with the same assay you are
using to run some patient samples that you have assayed. Once again both
labs should assay the same controls and calibrators as "unknowns" in these
runs.
Using this strategy, and working closely with another lab and the reagent
supplier, we were able to convince the company that there was a calcium
calibration problem.
I hope this helps.
Michael Peake
(retired)
Biochemistry Department
Flinders Medical Centre
Bedford Park
South Australia
Australia
(618) 8443 3332
Email: [log in to unmask]
-----Original Message-----
From: Clinical biochemistry discussion list
[mailto:[log in to unmask]] On Behalf Of Dr Maria Fitzgibbon
Sent: Saturday, 23 February 2013 1:53 AM
To: [log in to unmask]
Subject: Abbott users - serum calcium
Importance: High
We have unearthed an issue which appears to relate to the higher point in
the 2-point Abbott calibration in the calcium assay. Where our IQC and EQA
have been acceptable we noticed an alarming number of patients with
borderline high serum calciums. We have stopped using the assay and on look
back at our data there has been an upward trend towards the latter part of
2012, but with a recent unacceptable shift in the past month.
Anyone else have this problem?
Best w, Maria
Dr Maria Fitzgibbon, FRCPath,
Consultant Clinical Biochemist,
Head of Department of Clinical Biochemistry & Diagnostic Endocrinology,
Mater Misericordiae University Hospital, Eccles St, Dublin 7.
Tel: 0035318032423
email: [log in to unmask]
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