Hi Paul,
We have run ammonias successfully for many years on LiH samples on a Vitros platform. You have to spin the sample hard enough to make platelet poor plasma (there is a lot of ammonia in platelets). You can check you are acheiving this by doing plasma platelet counts on the haematology analyser. The routine spin for U and E is usually not hard enough to get rid of the platelets.
Kind regards
Dr David Cowley
Director of Chemical Pathology
Mater Health Services Brisbane
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From: Clinical biochemistry discussion list [[log in to unmask]] On Behalf Of Paul Thomas [[log in to unmask]]
Sent: Wednesday, 20 June 2012 10:17 PM
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Subject: ammonia assay Roche platforms
Roche recommend K-EDTA plasma for ammonia assays, does anybody have any experience or evaluation data on lithium heparin plasma? It would be much easier to run it on the U&E sample.
Thank you for your help
Paul
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