I remember I saw the similar problem caused by beta-mercaptoethanol.
Kevin
On Mon, Mar 12, 2012 at 1:29 PM, Artem Evdokimov
<[log in to unmask]> wrote:
> Could be one of those weird behaviors displayed by detergents where cloud
> point anomalously changes with temperature...
>
> Artem
>
> On Mar 12, 2012 1:11 PM, "Min-Kyu Cho" <[log in to unmask]> wrote:
>>
>> I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol,
>> 0.02%
>> NaN3.
>>
>> Yes, I agree I should check CD melting curve to see temperature preference
>> of my protein.
>>
>> Min-Kyu
>>
>> | -----Original Message-----
>> | From: Kevin Jin [mailto:[log in to unmask]]
>> | Sent: Monday, March 12, 2012 11:16 AM
>> | To: Min-Kyu Cho
>> | Cc: [log in to unmask]
>> | Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4
>> | oC
>> |
>> | Which kind of buffer you use? If it is Tris, then temperature change
>> will
>> | cause pH change.
>> |
>> | Actually, this is a good way for crystallization.
>> |
>> | Kevin
>> |
>> | On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <[log in to unmask]>
>> wrote:
>> | > Hi all,
>> | >
>> | > I have a homotetrameric coiled-coil domain sample with 45aa per each.
>> | > While I store this sample at 4oC, the sample looks clear w/o any
>> | > particles. But when I took out the sample to my bench at r.t, I can
>> | > see there are precipitates (as stack of needle like particles) at the
>> | > bottom of the tube after several hours. Interestingly, when I put it
>> | > back into 4oC fridge, the precipitates disappeared and the solution
>> | turned into clear again.
>> | >
>> | > Does anyone have knowledge of such behavior of any protein? I
>> | > appreciate any information related.
>> | >
>> | > Min-Kyu
|