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I remember I saw the similar problem caused by beta-mercaptoethanol. Kevin On Mon, Mar 12, 2012 at 1:29 PM, Artem Evdokimov <[log in to unmask]> wrote: > Could be one of those weird behaviors displayed by detergents where cloud > point anomalously changes with temperature... > > Artem > > On Mar 12, 2012 1:11 PM, "Min-Kyu Cho" <[log in to unmask]> wrote: >> >> I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol, >> 0.02% >> NaN3. >> >> Yes, I agree I should check CD melting curve to see temperature preference >> of my protein. >> >> Min-Kyu >> >> | -----Original Message----- >> | From: Kevin Jin [mailto:[log in to unmask]] >> | Sent: Monday, March 12, 2012 11:16 AM >> | To: Min-Kyu Cho >> | Cc: [log in to unmask] >> | Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4 >> | oC >> | >> | Which kind of buffer you use? If it is Tris, then temperature change >> will >> | cause pH change. >> | >> | Actually, this is a good way for crystallization. >> | >> | Kevin >> | >> | On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <[log in to unmask]> >> wrote: >> | > Hi all, >> | > >> | > I have a homotetrameric coiled-coil domain sample with 45aa per each. >> | > While I store this sample at 4oC, the sample looks clear w/o any >> | > particles. But when I took out the sample to my bench at r.t, I can >> | > see there are precipitates (as stack of needle like particles) at the >> | > bottom of the tube after several hours. Interestingly, when I put it >> | > back into 4oC fridge, the precipitates disappeared and the solution >> | turned into clear again. >> | > >> | > Does anyone have knowledge of such behavior of any protein? I >> | > appreciate any information related. >> | > >> | > Min-Kyu