Hi Anita,
I have recently performed it following this protocol I found in the Internet:
http://wolfson.huji.ac.il/purification/Protocols/PAGE_Acidic.html
I hope this helps! Good luck,
Federica
--------------
Federica Basilico
Ph.D. student
Department of Experimental Oncology
European Institute of Oncology
Via Adamello 16
20139 Milan (Italy)
anita p ([log in to unmask]) wrote:
>
> Hi All,
> Has anyone run a native gel for proteins at pI>8 .
> I want to pour my own native gel. Do I run a discontinuous page or a
> continuous one?? Please help with regards to the buffer system to be used,
> and the dye to be used.
> With regards
> Rashmi
>
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