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Hi Anita, I have recently performed it following this protocol I found in the Internet: http://wolfson.huji.ac.il/purification/Protocols/PAGE_Acidic.html I hope this helps! Good luck, Federica -------------- Federica Basilico Ph.D. student Department of Experimental Oncology European Institute of Oncology Via Adamello 16 20139 Milan (Italy) anita p ([log in to unmask]) wrote: > > Hi All, > Has anyone run a native gel for proteins at pI>8 . > I want to pour my own native gel. Do I run a discontinuous page or a > continuous one?? Please help with regards to the buffer system to be used, > and the dye to be used. > With regards > Rashmi >