Ditto to Poul's advice.
I've had many many many cases where crystals diffract poorly (or not at all) on home sources only to show excellent diffraction at a synchrotron. (Whether or not a home source is properly calibrated is probably the biggest issue, but that's for another discussion).
On Jan 26, 2012, at 8:33 AM, Theresa H. Hsu wrote:
> Dear crystallographers
>
> I have a protein of 90 kDa forming dimers. Crystals formed with microbatch and vapor diffusion method in 24 hours but no diffraction at home source. Dissolved crystals was confirmed to be the protein with mass spec.
>
> Any suggestions to improve diffraction would be welcome.
>
> Thanking you in advance.
>
> Theresa
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Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
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