It depends a lot on which home source and which synchrotron, there are
enormous differences. Goettingen is uniquely well placed because we can
reach four synchrotrons in a few (3-7) hours by high speed train and in
theory at least five more with a longer train journey, trains are very
convenient for transporting crystals. Two of these synchrotrons do not
give a higher resolution than our home system, but at least they can
vary the wavelength. However if we think we can see at least two
reflections at home, of course we take the crystal to a (suitable)
synchrotron.
George
On 01/26/2012 04:54 PM, Francis E Reyes wrote:
> Ditto to Poul's advice.
>
> I've had many many many cases where crystals diffract poorly (or not at all) on home sources only to show excellent diffraction at a synchrotron. (Whether or not a home source is properly calibrated is probably the biggest issue, but that's for another discussion).
>
>
>
> On Jan 26, 2012, at 8:33 AM, Theresa H. Hsu wrote:
>
>> Dear crystallographers
>>
>> I have a protein of 90 kDa forming dimers. Crystals formed with microbatch and vapor diffusion method in 24 hours but no diffraction at home source. Dissolved crystals was confirmed to be the protein with mass spec.
>>
>> Any suggestions to improve diffraction would be welcome.
>>
>> Thanking you in advance.
>>
>> Theresa
>
>
> ---------------------------------------------
> Francis E. Reyes M.Sc.
> 215 UCB
> University of Colorado at Boulder
>
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