On Wed, Nov 23, 2011 at 7:57 AM, Ian Tickle <[log in to unmask]> wrote:
> On 23 November 2011 07:54, Careina Edgooms <[log in to unmask]> wrote:
>> I have a question about a 2F0-Fc difference map that I calculated with Refmac.
>
> On 23 November 2011 15:40, Nat Echols <[log in to unmask]> wrote:
>> The negative density around Met S could be radiation damage.
>
> But you wouldn't expect to see -ve density in the 2Fo-Fc map from
> radiation damage right? The Fo-Fc map for sure.
I assumed that's what the original poster meant.
(and I apologize for the redundant comments, GMail groups messages by
subject, so every time someone changes the subject line, it becomes a
new thread, which I usually miss. That said, I thought after reading
Garib's Refmac paper published earlier this year that it was now using
distance-based B-factor restraints, instead of bond connectivity - is
this correct or did I misunderstand?)
-Nat
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