Becky
Do you have the nitrogen stream co-linear with the capillaries, with the
specimen near the capillary end and therefore near the nitrogen stream
exit? This might help to minimise turbulence - though nowadays other
bits of kit fight to occupy this space.
Regards
Colin
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
R
> Conners, Biochemistry
> Sent: 17 February 2011 17:03
> To: [log in to unmask]
> Subject: [ccp4bb] Freezing crystals in a contained system
>
> Dear all,
>
> We are working on a Category 3 protein which must be contained so we
> have
> our crystals mounted in a loop and then covered with a plastic Mitegen
> cover which is glued in place. We're currently collecting at room
> temperature, but wondered if anyone has any experience of using a
> contained
> system at low temperatures? Any attempts I've had so far at freezing
> through either the plastic or a glass capillary have resulted in
> formation
> of ice on the surface so it is not even possible to see the crystal to
> centre it.
>
> Best wishes,
>
> Becky
>
> -------------------------------------------------
> Dr Becky Conners
> School of Biochemistry
> University of Bristol, UK
>
> http://www.bris.ac.uk/biochemistry/brady
> [log in to unmask]
> 0117 3312149
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