 |
 |
Becky Do you have the nitrogen stream co-linear with the capillaries, with the specimen near the capillary end and therefore near the nitrogen stream exit? This might help to minimise turbulence - though nowadays other bits of kit fight to occupy this space. Regards Colin > -----Original Message----- > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of R > Conners, Biochemistry > Sent: 17 February 2011 17:03 > To: [log in to unmask] > Subject: [ccp4bb] Freezing crystals in a contained system > > Dear all, > > We are working on a Category 3 protein which must be contained so we > have > our crystals mounted in a loop and then covered with a plastic Mitegen > cover which is glued in place. We're currently collecting at room > temperature, but wondered if anyone has any experience of using a > contained > system at low temperatures? Any attempts I've had so far at freezing > through either the plastic or a glass capillary have resulted in > formation > of ice on the surface so it is not even possible to see the crystal to > centre it. > > Best wishes, > > Becky > > ------------------------------------------------- > Dr Becky Conners > School of Biochemistry > University of Bristol, UK > > http://www.bris.ac.uk/biochemistry/brady > [log in to unmask] > 0117 3312149