Hi,
you did not mention anything about your protein, but if it shows a metal
dependency, than different amounts of the metal ion might changen the overall
charge and influence the interactions with the ion exchanger. There might be
also a modification of an aminoacid f.e. decarboxylation of an aspartate or
glutamate.
Christian
Am Freitag 18 Februar 2011 18:13:36 schrieb Ulli Hain:
> Hi, I was wondering if anyone had possible explanations for a
> recombinantly expressed soluble protein that runs as 2 equal, slightly
> overlapping peaks on a cation exhanger but as one peak on a size exclusion
> column and same electrophoretic mobility on SDS-PAGE. -Ulli
>
> Adelaide Ulricke Hain
> PhD Candidate
> Johns Hopkins Bloomberg School of Public Health
> Department of Biochemistry and Molecular Biology
> 615 North Wolfe Street
> Baltimore, MD 21205
>
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