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Hi, you did not mention anything about your protein, but if it shows a metal dependency, than different amounts of the metal ion might changen the overall charge and influence the interactions with the ion exchanger. There might be also a modification of an aminoacid f.e. decarboxylation of an aspartate or glutamate. Christian Am Freitag 18 Februar 2011 18:13:36 schrieb Ulli Hain: > Hi, I was wondering if anyone had possible explanations for a > recombinantly expressed soluble protein that runs as 2 equal, slightly > overlapping peaks on a cation exhanger but as one peak on a size exclusion > column and same electrophoretic mobility on SDS-PAGE. -Ulli > > Adelaide Ulricke Hain > PhD Candidate > Johns Hopkins Bloomberg School of Public Health > Department of Biochemistry and Molecular Biology > 615 North Wolfe Street > Baltimore, MD 21205 >