Dear Tim,
I'd dare to say that SeMet labelling in mammalian cells is in
fact routine (at least in our lab, and others' around the
world) for quite a while :-)
Since you mentioned CHO LecR cells, one can find references as
early as 1994 from Wayne Hendrickson's lab (PMID: 7922031) and
many others followed. More recently, people have moved to
transient expression and probably the easiest labelling method
for HEK293 cells can be found in PMID: 17001101.
Best wishes,
radu
------------------------------------------
A. Radu Aricescu, PhD
University of Oxford
Wellcome Trust Centre for Human Genetics
Division of Structural Biology
Roosevelt Drive, Oxford OX3 7BN
United Kingdom
Phone: +44-1865-287564
Fax: +44-1865-287547
---- Original message ----
>Date: Tue, 23 Mar 2010 13:39:09 +0100
>From: CCP4 bulletin board <[log in to unmask]> (on behalf
of Jovine Luca <[log in to unmask]>)
>Subject: Re: [ccp4bb] update on SeMet production
>To: [log in to unmask]
>
>Dear Tim,
>
>Although it is not routine, it can be done in mammalian cells
too! For example, have a look at this:
>
>
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.p
df
>
>HTH,
>
>Luca
>
>-------------------------------------------------------------
---
>Luca Jovine, Ph.D.
>Karolinska Institutet
>Department of Biosciences and Nutrition & Center for
Biosciences
>Hälsovägen 7, SE-141 57 Huddinge, Sweden
>Voice: +46.(0)8.6083-301 FAX: +46.(0)8.6081-501
>E-mail: [log in to unmask]
>W3: http://jovinelab.org
>-------------------------------------------------------------
---
>
>On Mar 23, 2010, at 1:25 PM, Tim Gruene wrote:
>
>> Dear all,
>>
>> since I am currenty preparing a lecture on crystallography
I am wondering about
>> the status quo of the production of SeMet proteins.
>> In 2003, if I remember correctly, it was possible to
express SeMet proteins in
>> E.coli and insect cells.
>>
>> Has this been extended to other systems, and if so, which
ones?
>>
>> Thanks a lot,
>> Tim
>>
>> --
>> --
>> Tim Gruene
>> Institut fuer anorganische Chemie
>> Tammannstr. 4
>> D-37077 Goettingen
>>
>> GPG Key ID = A46BEE1A
>>
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