Xe is also an excellent anomalous scatterer at Cu Ka wavelength. It is
particularly good for SIRAS phasing since Xe derivative is usually highly
isomorphous to the native crystals.
Hong
_____________________
Hong Zhang, Ph.D.
Department of Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, Texas 75390-8816
Phone: 214-645-6372
Fax: 214-645-5948
Web: http://hhmi.swmed.edu/Labs/hz/
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Georg
Zocher
Sent: Monday, October 26, 2009 3:31 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] SAD phasing at home source
Dear James,
A little bit more "old school" but you might also try Uranium, Samarium
and Gadolinium.
In principle, take a look what compounds you have in your HA box and
afterwards check anomalous signal for example at
http://skuld.bmsc.washington.edu/scatter/AS_form.html
Best regards,
Georg
james09 pruza schrieb:
> Dear All,
>
> I need some suggestions regarding the SAD phasing using home source.
> What the the most commonly used HA for SAD phasing for Cu anode.
> All suggestions are welcome.
> Thanks.
>
> James
--
Universität Tübingen
Interfakultäres Institut für Biochemie
Dr. Georg Zocher
Hoppe-Seyler-Str. 4
72076 Tuebingen
Germany
Fon: +49(0)-7071-2973374
Mail: [log in to unmask]
http://www.ifib.uni-tuebingen.de
|