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Xe is also an excellent anomalous scatterer at Cu Ka wavelength. It is particularly good for SIRAS phasing since Xe derivative is usually highly isomorphous to the native crystals. Hong _____________________ Hong Zhang, Ph.D. Department of Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Dallas, Texas 75390-8816 Phone: 214-645-6372 Fax: 214-645-5948 Web: http://hhmi.swmed.edu/Labs/hz/ -----Original Message----- From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Georg Zocher Sent: Monday, October 26, 2009 3:31 PM To: [log in to unmask] Subject: Re: [ccp4bb] SAD phasing at home source Dear James, A little bit more "old school" but you might also try Uranium, Samarium and Gadolinium. In principle, take a look what compounds you have in your HA box and afterwards check anomalous signal for example at http://skuld.bmsc.washington.edu/scatter/AS_form.html Best regards, Georg james09 pruza schrieb: > Dear All, > > I need some suggestions regarding the SAD phasing using home source. > What the the most commonly used HA for SAD phasing for Cu anode. > All suggestions are welcome. > Thanks. > > James -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: [log in to unmask] http://www.ifib.uni-tuebingen.de